ITC

Isothermal Titration Calorimetry (ITC) is for measuring biomolecular interactions.  ITC simultaneously determines all binding parameters (n, K, ΔH and ΔS) in a single experiment – information that cannot be obtained from any other method.

 

When substances bind, heat is either generated or absorbed.  ITC is a thermodynamic technique that directly measures the heat released or absorbed during a biomolecular binding event.  Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment.  Because ITC goes beyond binding affinities and can elucidate the mechanism of the molecular interaction, it has become the method of choice for characterizing biomolecular interactions.